(A) First strand cDNA is initiated by priming with an oligo dT primer
Template Switch Oligo. In the first step, template switching reverse transcription reaction. Web this primer, in conjunction with a template switching oligo (tso), generates cdnas containing adaptor sequences at.
(A) First strand cDNA is initiated by priming with an oligo dT primer
Web the tso or (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the. In the first step, template switching reverse transcription reaction. Web this primer, in conjunction with a template switching oligo (tso), generates cdnas containing adaptor sequences at. Web this 5′ race protocol contains two steps. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse.
Web this primer, in conjunction with a template switching oligo (tso), generates cdnas containing adaptor sequences at. Web this primer, in conjunction with a template switching oligo (tso), generates cdnas containing adaptor sequences at. In the first step, template switching reverse transcription reaction. Web the tso or (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the. Web this 5′ race protocol contains two steps. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse.